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SP Kit(Broad Spectrum) (SP-0022)

SP Kit(Broad Spectrum) (SP-0022)

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SP Kit(Broad Spectrum) 

英文名称SP Kit(Broad Spectrum)
中文名称广谱SP免疫组化检测试剂盒(用于检测兔、大鼠、小鼠和豚鼠来源的一抗)
别    名Streptavidin-Peroxidase Immunohistochemical staining kit; Histostain-Plus Kit (HRP, Broad Spectrum)
保存条件Store at 2-8 ℃
产品介绍Histostain-Plus kits use 2nd generation LAB-SA technology and are ideal for general immunohistochemical applications. Histostain-Plus kits offer superior sensitivity without compromising background. They are designed to detect mouse, rabbit, rat, or guinea pig primary antibodies that are bound to human tissue or cell antigens. Histostain-Plus kits are excellent for frozen and formalin-fixed, paraffin-embedded tissue sections, cell smears, etc. These kits are very easy to use: all components are liquid (except Fast-Red) and come in dropper bottles. No reconstitution, no pipetting, no measuring is necessary, and the enzyme is conjugated to streptavidin to eliminate the preincubation complexing step. DAB chromogen yields a brown stain, AEC yields a deep red stain, and Fast-Red yields a bright red stain.
Reactivity: Mouse, Rabbit, Rat, Guinea Pig
Applications: Immunohistochemistry (IHC)




即用型SP(Streptavidin-Peroxidase)免疫组化检测试剂盒是利用LAB-SA第二代技术生产的免疫组化检测试剂,最大特点是高灵敏度,低背景染色,直接滴加,操作方便,孵育时间短,适用于冷冻切片、石蜡切片及固定的细胞涂片。
广谱是指可用于检测兔、大鼠、小鼠和豚鼠来源的一抗

SP试剂盒内容

无色试剂:内源性过氧化物酶阻断剂
试剂A:封闭用正常山羊血清工作液
试剂B:生物素标记二抗工作液:生物素标记羊抗兔、大鼠、小鼠和豚鼠IgG
试剂C:辣根酶标记链酶卵白素工作液


温馨提示:
1. 本系列检测试剂盒适用于绝大数组织,但对于内源性生物素含量比较丰富的组织(如肾脏、肝脏等),应选用非生物素检测系统,或用avdin封闭。
2. 工作液:如以每张切片加入1滴(约50μl)计算,3ml可做60张切片,6ml可做120张切片。
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