FITC
| 英文名称 | FITC |
| 中文名称 | 荧光标记染料FITC/异硫氰酸荧光素酯 |
| 别 名 | Fluorescein isothiocyanate isomer I; 3',6'-dihydroJNC-5-isothiocyanato-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one; 5-FITC; FITC-I |
| 保存条件 | Store at -20℃, protect from light. |
| 产品介绍 | Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. A succinimidyl-ester functional group attached to the fluorescein core, creating "NHS-fluorescein", forms another common amine reactive derivative that has much greater specificity toward primary amines in the presence of other nucleophiles. FITC has excitation and emission spectrum peak wavelengths of approximately 495 nm/519 nm, giving it a green color. Like most fluorochromes, it is prone to photobleaching. Because of the problem with photobleaching, derivatives of fluorescein such as Alexa 488 and DyLight 488 have been tailored for various chemical and biological applications where greater photostability, higher fluorescence intensity, or different attachment groups are needed. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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