Dithiothreitol (DTT)
| 英文名称 | Dithiothreitol (DTT) |
| 中文名称 | 二硫苏糖醇(BR，99%) |
| 别 名 | DL-Dithiothreitol; DTT; (2S,3S)-1,4-Bis(sulfanyl)butane-2,3-diol; (2S,3S)-1,4-Dimercaptobutane-2,3-diol; D-threo-1,4-Dimercaptobutane-2,3-diol; D-threo-1,4-Dimercapto-2,3-butanediol; 1,4-Dithio-D-threitol; Cleland's reagent; Reductacryl; |
| 保存条件 | Store at 2-8℃. Keep dry. |
| 产品介绍 | "DTT is frequently used to reduce the disulfide bonds of proteins and, more generally, to prevent intramolecular and intermolecular disulfide bonds from forming between cysteine residues of proteins. DTT is oftentimes used along with sodium dodecylsulfate in SDS-PAGE to further denature proteins by reducing their disulfide bonds to allow for better separation of proteins during electrophoresis. DTT is used as a reducing or ""deprotecting"" agent for thiolated DNA. The terminal sulfur atoms of thiolated DNA have a tendency to form dimers in solution, especially in the presence of oJNCgen. " Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. CAS号：3483-12-3 分子式：C4H10O2S2 分子量：154.25 MDL：MFCD00004877 级别：BR 纯度：99% 外观/性状：白色结晶性粉末。 溶解性：易溶于水,也溶于乙醇、丙酮、乙酸乙酯、氯仿和乙醚。 敏感性：微吸潮。在空气中缓慢氧化。建议：储存液/工作液必须现配现用，剩余液体需丢弃。 产品描述：二硫苏糖醇是一种强效的小分子有机还原剂，将-SH基保持在还原态，常用以还原蛋白和多肽中的二硫键，或更普遍用以防止蛋白半胱氨酸残基形成分子内和分子间的二硫键。DTT还具有抗氧化作用，对一些隐藏的二硫键（溶剂无法渗透发挥作用），DTT可借助变性条件下来还原二硫键，如高温或钠离子变性剂，如6 M盐酸胍、8 M尿素或1%SDS。 与β-巯基乙醇相比，二者作用相似，但DTT的刺激性和毒性都低于β-巯基乙醇，且粉末的稳定性也高于β-巯基乙醇。工作pH范围：pH 6.5-9.0，最佳范围：pH 7.1-8.0。蛋白巯基还原实验中常用工作浓度：1-10mM。 |
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