ADH
英文名称 | ADH |
中文名称 | 乙醇脱氢酶 |
别 名 | Alcohol dehydrogenase; Alcohol Dehydrogenase from yeast, Alcohol:NAD+ oxidoreductase; Alcohol Dehydrogenase from Saccharomyces cerevisiae; |
保存条件 | Store at -20℃. |
产品介绍 | 级别:BR CAS号:9031-72-5 mol wt: 141-151kDa MDL:MFCD00051089 form: lyophilized powder (contains ≤ 2% citrate buffer salts),≥300 units/mg protein. purified by: crystallization Featured Industry: Diagnostic Assay Manufacturing. Unit Definition: One unit will convert 1.0 μmole of ethanol to acetaldehyde per min at pH 8.8 at 25 °C. Preparation Note: Dissolves in water at a concentration of 1 mg/mL to form a clear to slightly hazy, colorless to faintly yellow colored solution. solubility: H2O: soluble 1.0 mg/mL, clear to slightly hazy, colorless to faintly yellow. 用途:分子量为 141kDa 的四聚体包含了 4 个相同的亚基。每个亚基的活性位点都含有一个锌原子。每个活性位点还含有 2 个反应性巯基以及一个组氨酸残基。 等电点:5.4-5.8 最适 pH:8.6-9.0 底物:酵母乙醇脱氢酶与乙醇最易反应,它的反应活性随着醇体积增大或减小而降低。与支链醇和仲醇的反应活性也非常低。 KM(乙醇)= 2.1×10-3M KM(甲醇)= 1.3×10-1M KM(异丙醇)= 1.4×10-1M 抑制剂:能与游离巯基反应的化合物,包括 N-烷基马来酰亚胺和碘乙酰胺。 |
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